| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Abundance and diversity of ammonia-oxidizing bacteria in relation to ammonium in a Chinese shallow eutrophic urban lake
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| Autores: |
Qiu,Shanlian
Chen,Guoyuan
Zhou,Yiyong
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| Data: |
2010-03-01
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| Ano: |
2010
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| Palavras-chave: |
Ammonia-oxidizing bacteria
Abundance
AmoA
Diversity
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| Resumo: |
The measures of most-probable-number and restriction fragment length polymorphism analysis were used to analyze the abundance and diversity of ammonia-oxidizing bacteria in sediment of a Chinese shallow eutrophic urban lake (Lake Yuehu). Among the 5 sampling sites, ammonia concentration in interstitial water was positively proportional not only to the content of organic matter, but also to ammonia-oxidizing bacteria numbers (at a magnitude of 10(5) cells g-1 dry weight) in sediment significantly. Furthermore, the diversity of ammonia-oxidizing bacteria were determined by means of PCR primers targeting the amoA gene with five gene libraries created and restriction pattern analysis. The 13 restriction patterns were recorded with 4 ones being common among all sampling sites. The 8 restriction patterns including 4 unique ones were found at the site with the highest NH4+ concentrations in interstitial water, while, there were only common patterns without unique ones at the site with the lowest NH4+ concentrations in interstitial water. Phylogenetic analysis showed that the amoA fragments retrieved belong to Nitrosomonas oligotropha & ureae lineage, N. europaea lineage, N. communis lineage and Nitrosospira lineage, most of which were affiliated with the genus Nitrosomonas. The N. oligotropha & ureae-like bacteria were the dominant species. Thus, the abundance and diversity of sediment AOB is closely linked to ammonium status in eutrophic lakes.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100031
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822010000100031
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.41 n.1 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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